true C (3-6 months) <a href='https://brc.riken.jp/mus/pcr00462'>Genotyping protocol -PCR-</a> Roche Group Chugai Pharmaceutical Co., Ltd. B6.Cg-Mak<tm1Yshk>/YshkRbrc In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Sibling mating. Homozygous male are fertile, but exhibit slight reduction in litter size and sperm motility in vitro. MAK Knockout mice B6 B6.Cg-Mak<tm1Yshk>. Mak, male germ cell-associated kinase gene expresses specifically in testicular germ cells. Mak deficient mice show no gross abnormalities, and most of the mice were fertile. However, the litter size derived homozygous mutant males and their sperm motility in vitro were mildly reduced compared to those of normal mice. This strain is useful for elucidation of the function of Mak gene in spermatogenesis. ホモMAK KO オスはfertileだが、個体によっては精子の運動性が極端に悪いものがある。 Sibling mating.Homozygous male are fertile, but exhibit slight reduction in litter size and sperm motility in vitro. mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse male germ cell-associated kinase (MAK) genomic DNA 1998年、日本ロシュ(株)研究所で作製。 日本クレアのC57BL/6N-Jclとbackクロスを9回行ったものを理研BRCに導入。 MAK Knockout mice B6 MAK Knockout mice B6 C（3〜6か月） ヘテロマウスはAホモマウス　メスはA、オスはA(個体によってはB） 1998年、日本ロシュ(株)研究所で作製。日本クレアのC57BL/6N-Jclとbackクロスを9回行ったものを理研BRCに導入。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 日本ロシュ(株)研究所 Cell Biology Research TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] Developed by Dr. Shinkai at Kyoto University. A neo cassette was transferred into TT2 ES cells to replace exons 5-8 in the Mak gene. The mutant mice were backcrossed to C57BL/6NJcl for 9 times. RBRC00462 Frances Ford Frances Ford ヘテロマウスはA ホモマウス　メスはA、オスはA(個体によってはB） 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。<br>研究成果の公表にあたって謝辞の表明を必要とする。